Table 3. Kinetic profiles of reported high-affinity ETS-DNA interactions.
ETS domain | ka, M−1 s−1 | kA, s−1 | KD, nM | [salt], mM | Reference |
---|---|---|---|---|---|
PU.1 | (3.2 ±0.1) × 104 | (5.9 ±0.2) × 10−4 | 18 ± 1.5 | 250 (Na+) | This work |
(4.9 ± 1.2) × 105 | (8.5 ± 2.0) × 10−2 | 170 ± 5 | 650 (Na+) | Pióet al.21a | |
Ets-1 | 14 × 108 | 1.6 × 10−2 | 0.0085 ± 0.0007 | 65 (K+) + 6 Mg2+ | Jonsen et al.42b |
TEL (ETV6) | 8.7 × 107 | ~0.2 | 2.3 ± 0.4 | 50 (K+) + 6 (Mg2+) | Green et al.41b |
No nonspecific DNA was used in this SPR study.
kd determined by EMSA; ka inferred by Equation 4.