Table 3. Kinetic profiles of reported high-affinity ETS-DNA interactions.
The values excerpted from the literature are for a construct most closely corresponding to the minimal ETS domains (i.e., minus autoinhibitory modules) that represent an appropriate counterpart to the PU.1 ETS domain. In cases where no error is stated in the table, none was reported.
| ETS domain | ka, M−1 s−1 | kA, s−1 | KD, nM | [salt], mM | Reference |
|---|---|---|---|---|---|
| PU.1 | (3.2 ±0.1) × 104 | (5.9 ±0.2) × 10−4 | 18 ± 1.5 | 250 (Na+) | This work |
| (4.9 ± 1.2) × 105 | (8.5 ± 2.0) × 10−2 | 170 ± 5 | 650 (Na+) | Pióet al.21a | |
| Ets-1 | 14 × 108 | 1.6 × 10−2 | 0.0085 ± 0.0007 | 65 (K+) + 6 Mg2+ | Jonsen et al.42b |
| TEL (ETV6) | 8.7 × 107 | ~0.2 | 2.3 ± 0.4 | 50 (K+) + 6 (Mg2+) | Green et al.41b |
a
No nonspecific DNA was used in this SPR study.
b
kd determined by EMSA; ka inferred by Equation 4.