Fig. 6.

Quantitative analysis of chromocenter condensation after pharmacological PARP inhibition. Statistical analysis (b) after signal quantification and three dimensional volume analysis on laser scan microscopy images (a) reveals a significant reduction of the median nuclear volume occupied by intense major satellite DNA signals and indicates a lower general degree of chromocenter condensation (I; n=59–87 individually imaged sperm per treatment group and experiment, in 3 independent hybridization experiments, significance was determined on values obtained in 3 individual hybridization experiments, and calculated by students t-test followed by Bonferroni correction for multiple measurements; mean values are indicated in red). c. Frequency distribution of nuclear volume of major satellite FISH signals condensed above signal detection limit per sperm (left panels; bin width = 2, median binned value is indicated by red arrows) (n= 58–87 sperm were analyzed per treatment group; and the analysis was repeated in three independent experiments. Mann Whitney tests indicate significant differences between treatment groups, ****, p<0.0001). Right panel shows the numbers of individual FISH foci visible for major satellite DNA probe in sperm from control and PARP inhibitor (PJ34) treated animals