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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Glia. 2010 Sep;58(12):1407–1414. doi: 10.1002/glia.21016

Figure 4.

Figure 4

Assays of oligodendrocyte proliferation and apoptosis in E16.5 spinal cords. A–B. Double immunofluorescent staining with anti-BrdU and anti-Olig2. Double-stained cells are represented by arrows. C–D Double immunostaining with anti-pCaspase 3 and anti-Sox10. Green and red arrowheads represent oligodendrocytes and apoptotic cells, respectively. Scale bar, 100 μm. (E) The percentage of BrdU labeled Olig2+ cell at E16.5. More than 400 Olig2-positive and BrdU/Olig2 double positive cells from 4 different sections were counted under 10X magnification (*P<0.01).