Figure 4. Kinetic of HAC1 mRNA splicing by chimeric Ire1.
AWY14 or AWY19 strains harboring the indicated plasmids were cultured in medium containing galactose for 4 hr. status of HAC1 mRNA splicing was monitored upon exposing to β-ME for indicated time. The efficiency of the splicing was analyzed by Scion Image program. M: 1 kb plus ladder.