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. 2008 Apr 15;283(24):16446–16454. doi: 10.1074/jbc.M802272200

FIGURE 2.

FIGURE 2.

Consequences of OS-9 up-regulation on secretion and degradation of NHK and on secretion of α 1-antitrypsin. A, radiolabeled NHK has been immunoisolated after the indicated chase times from detergent extract of cells with normal (lanes 1–3) or elevated levels of OS-9.1 (lanes 4 – 6) or OS-9.2 (lanes 7–9). Note the co-precipitation of ectopic OS-9.1 and ectopic OS-9.2. B, quantification of intracellular NHK (n = 4). C, secretion of labeled NHK from cells with the normal (lanes 1–3) or elevated content of OS-9.1 (lanes 4 – 6) or OS-9.2 (lanes 7–9). D, quantification of C (n = 3). E, same as A for α1-antitrypsin. F, quantification of E. G, same as C for α1-antitrypsin. H, quantification of G. I, NHK co-precipitates with OS-9.1 (lanes 4 – 6) and OS-9.2 (lanes 7–9). L, α1-antitrypsin does not co-precipitate with OS-9 variants.