Fig. 1.

In human CC, Fr.II T_regs expand in a cancer stage–dependent manner. (A) Representative dot plots depicting flow cytometry gating of five T cell fractions: three of which are Foxp3⁺ and two are Foxp3⁻. The Foxp3⁺ cells are labeled as Fr.I (black, CD45RA⁺Foxp3^int), Fr.II (red, CD45RA⁻Foxp3^high), and Fr.III (blue, CD45RA⁻Foxp3^int). Non-Foxp3 T cells can be divided into naïve CD45RA⁺Foxp3⁻ (green) and memory CD45RA⁻Foxp3⁻ (purple). Cells were pregated on live CD4⁺CD8⁻ cells. (B) Compiled frequencies of T_reg subtypes among CD4 T cells frommarginal or tumor tissue as determined by FACS analysis (n = 13). (C) Compiled frequencies of T_reg subtypes among CD4 T cells from PBMCs of HD or CC patients. HD, n = 23; CC, n = 94. (D) CC PBMC T_reg subtype frequencies according to the cancer stage of each CC patient as defined by the International Union for Cancer Control. Fr.I: white circles, HD; white squares, CC; Fr.II: red circles, HD; red squares, CC; Fr.III: blue circles, HD; blue squares, CC. Statistics are found in table S2. Bar represents median. Each dot in (B) to (D) represents one patient; value was determined from an average of at least two separate FACS stainings.