Fig. 7.

Loss of RORγt, but not IL-17, restores T_reg anti-inflammatory function. (A) Frequency of MC progenitors (MCp) among total mononuclear cells (MNCs) isolated from the intestine of Rag^−/− mice and cultured without (n = 5) or with T_regs at 1:1 ratio from wild-type B6 (n = 6), APC^Δ468 (n = 5), or IL-17^−/− BM in APC^Δ468 (n = 6), or with RORγt^−/−xAPC^Δ468 (n = 5) mice. (B) Compiled values of percent MC degranulation sensitized (IgE) and cross-linked with anti-IgE (IgE + Ag) and incubated without (n = 10) or with T_regs from wild-type B6 (n = 16), APC^Δ468 (n = 15), IL-17^−/− BM in APC^Δ468 (n = 8), or RORγt^−/−xAPC^Δ468 (n = 10) mice. (C) Frequency of polyps after retro-orbital adoptive transfer of 5 × 10⁵ T_regs into 2.5-month-old APC^Δ468 mice. Polyp number was determined 3 weeks later. B6 (n = 5), APC^Δ468 (n = 4), RORγt^−/−xAPC^Δ468 (n = 3). (D) Representative dot plot of intracellular cytokines in T_regs derived from the spleen of wild-type B6 or RORγt^−/− mice. Purified T_regs were cultured without or with MCs for 5 days in the presence of IL-2 and stem cell factor (SCF). Cells were gated on live CD4⁺CD25⁺Foxp3⁺ cells (n = 3). Statistics are found in table S7.