Table 1.
Average speed (μm/second ± SD) | Distance index
|
||
---|---|---|---|
Range (%) | Average ± SD (%) | ||
G-clathrin | 3.73 ± 0.53 | 5.23–12.77 | 8.00 ± 1.78 |
Vectorial clathrin | 1.43 ± 0.66 | 25.73–85.73 | 63.34 ± 19.14 |
Stationary clathrin | <0.01 | N/A | N/A |
N/A, not applicable.
GFP–clathrin was imaged in live cells for 10 seconds using 36-millisecond exposures. All moving spots that persisted continuously as distinct structures for more than 5 seconds (56 spots from four cells) were tracked using the Metamorph ‘track points’ function, and the average speed, distance index and root mean square displacement were calculated in Excel. Stationary spots (173 in these images collected at the PM focal plane) had average speed values ≤0.01 μm/second. Distance index: the greatest separation distance achieved by a spot in a time stack divided by its total accumulated distance moved.