Figure 2.
The localization of different dsRBDs of DCL1 in living plant cells. A, Subcellular localization of DCL1-D1D2-YFP, DCL1-D2-YFP, and DCL1-D1-YFP. DCL1-D1D2-YFP and DCL1-D2-YFP localize in discrete nuclear foci, while DCL1-D1 shows diffused signals throughout the nucleoplasm. The root epidermal cells (top) and leaf epidermal cells (bottom) are shown. B, In transgenic plants coexpressing DCL1-D1-YFP/HYL1-CFP, DCL1-D2-YFP/HYL1-CFP, and DCL1-D1D2-YFP/HYL1-CFP, the nuclear bodies of DCL1-D1D2-YFP and DCL1-D2-YFP (green) fully colocalize with HYL1-CFP-containing d-bodies (magenta), while DCL1-D1-YFP shows no colocalization. Bars = 10 μm.