Figure 4.

A model for the recruitment of pri-miRNAs to d-bodies through functionally divergent dsRBDs of DCL1. MiRNA genes are transcribed by RNA polymerase II to generate pri-miRNAs. DCL1-D1 plays a role in pri-miRNA binding. In contrast, DCL1-D2 is involved in protein-protein interaction, thus guides DCL1 and its bound pri-miRNA to the d-bodies. The two N-terminal dsRBDs of HYL1 play similar roles to dsRBDs of DCL1. When the four DsRBDs bind together, they anchor the pri-miRNA molecule in place, and thus guarantee the accuracy of the dicing reaction of DCL1.