Table I. Elemental concentrations in a 1% agar/agarose solution.

All values are in µm. Shown are means (n = 3–4 measurements from the same batch). Different letters indicate significant differences among means in the column (P < 0.05; Tukey test). Boldface values indicate agar that was selected to study RSA responses to the deficiency of the nutrient in question. Al, Aluminum; Na; sodium.

Element		Al	B	Ca	Cu	Fe	K	Mg	Mn	Mo	N	Na	P	S	Zn
1/2 MS concentration	0	50	1,500	0.05	75	10,025a	750	50	0.5	11,400	0a	625	750a	15
Agar	Difco	2.2 ab	130 ab	380 c	0.22 ab	2.1 abc	730 ab	170 ab	0.13 e	0 ns	1,610 ab	2,270 b	5.9 abc	1,840 a	0.12 ab
	Duchefa A	1.8 ab	83 ab	340 d	0.07 ab	2.4 abc	110 abc	140 ab	0.10 f	0.012 ns	1,320 b	2,770 a	12 abc	1,620 b	0.02 b
	Duchefa B	3.6 ab	150 a	510 b	0.07 ab	1.9 abc	440 abc	390 a	0.46 c	0 ns	1,510 ab	2,070 c	29 abc	1,820 a	0.18 ab
	Roth	1.7 ab	0 b	84 g	0.04 ab	9.4 abc	170 abc	25 ab	0.07 g	0 ns	280 c	1,080 d	18 abc	690 e	0.13 ab
	Sigma A	45 a	0 b	550 a	0.08 ab	11 ab	27,860 a	320 ab	1.10 b	0 ns	1,830 a	2,050 c	300 a	1,090 d	0.12 ab
	Sigma B	3.1 ab	40 ab	510 b	0.46 a	4.1 abc	69 abc	56 ab	0.25 d	0 ns	180 c	870 e	25 abc	1,270 c	0.41 ab
	Sigma C	24 ab	41 ab	150 f	0.31 ab	21 a	290 abc	210 ab	1.70 a	0.005 ns	1,510 ab	2,800 a	110 ab	1,130 d	0.57 a
Agarose	Applichem	0.5 b	25 ab	7 h	0 b	0.5 bc	68 abc	1 b	0 h	0 ns	0 c	610 f	0.5 c	46 f	0.01 b
	Serva	4.3 ab	0 b	190 e	0 b	0.3 c	22 bc	60 ab	0 h	0 ns	0 c	76 g	6.4 abc	58 f	0.10 ab
	Sigma D	0.6 b	46 ab	11 h	0 b	0.9 abc	14 c	2 b	0.01 h	0 ns	310 c	780 e	1.8 bc	66 f	0.03 ab

^aNot considering that contained within the MES buffer (pH adjusted with KOH or NaOH).