Figure 3.

Clusters obtained by the K-means clustering algorithm, including annotation, of ESTs differentially regulated in cryptogein-treated tobacco leaves under different light regimes, obtained by cDNA-AFLP. Excised leaves were treated with either water (control [Co]) or cryptogein (Cr) and subsequently kept in the dark (D) or in the light (L) for 12 h. The numbers in parentheses indicate how many ESTs were identified corresponding to the same gene. ACC, aminocyclopropane-1-carboxylic acid; BURP, acronym for BNM2 (Brassica napus microspore-derived embryo), USP (unknown seed protein), RD22 (drought-induced protein), and PG1β (β-subunit of polygalacturonase isozyme1); CaM, calmodulin; CDK, cyclin-dependent kinase; elF, elongation factor; EREBP/ERF, ethylene response element binding protein/ethylene response factor; NB-ARC, nucleotide-binding adaptor shared by APAF-1 (apoptotic peptidase activating factor1), R proteins, and CED-4 (Caenorhabditis elegans cell death gene); OXI1, oxidative signal-inducible1; RING, really interesting new gene; SAM, S-adenosyl-l-Met.