Figure 13.

HY5 is required to up-regulate the HYH and UNE10 transcription factor genes in low R/FR. Col-0, hfr1-4/sics1-1, and hy5-215 seedlings were grown for 7 d in a L/D cycle (16/8 h) in high R/FR and then maintained in high R/FR or transferred to low R/FR for different times. The graphs show the relative expression levels in high (dashed lines) and low (solid lines) R/FR of HYH and UNE10 in the different genotypes. Each value is the mean ± sd of three biological replicates normalized to EF1α expression. Statistical significance between different backgrounds was assessed by means of two-way ANOVA followed by the Bonferroni posthoc test.