Table 3.
Phenolic Compounds in Navel Orange Peel
peak |
tR (m i n) |
[M + H]+/ [M - H]− (m/z) |
[A + H]+/[A - H]− and other NI ions (m/z) |
UV/vis Abs λmax (nm) |
identification |
---|---|---|---|---|---|
hydroxycinnamatesb | |||||
P1 | 7.17 | −/355 | −/209, 191, 163 | 230, 312 | p-coumarate |
P2 | 7.94 | −/355 | −/209, 191, 163 | 230, 314 | p-coumarate |
P3 | 8.58 | −/385 | −/209, 191, 163 | 238, 298 sh, 326 | ferulate |
P4 | 9.25 | −/355 | −/209, 191, 163 | 230, 314 | p-coumarate |
P5 | 9.54 | −/355 | −/209, 191, 163 | 230, 314 | p-coumarate |
P6 | 10.82 | −/355 | −/209, 191, 163 | 230, 314 | p-coumarate |
P7 | 11.18 | −/385 | −/209, 193, 191 | 238, 298 sh, 326 | ferulate |
P8 | 11.73 | −/385 | −/209, 193, 191 | 238, 298 sh, 328 | ferulate |
P9 | 12.56 | −/385 | −/209, 193, 191 | 238, 298 sh, 328 | ferulate |
P10 | 15.22 | −/355, 385 | −/209, 193, 191, 163 | 238, 298 sh, 330 | p-coumarate + ferulate |
P11 | 15.38 | −/385 | −/209, 193, 191 | 240, 298 h, 328 | ferulate |
hydroxycinnamic acids detected in the alkaline hydrolyzed extract | |||||
P12 | 22.30 | −/163 | 224, 310 | p-coumaric acida | |
P13 | 24.82 | −/223 | 236,324 | sinapic acida | |
P14 | 25.22 | −/193 | 236 sh, 324 | ferulic acida,c | |
glycosylated flavones (group I) | |||||
O1 | 15.30 | 595/– | 475, 355/– | 272, 334 | apigenin 6,8-di-C-glucoside |
O2 | 16.50 | 625/– | 505, 385/– | 256, 272, 348 | diosmetin 6,8-di-C-glucoside |
O5 | 23.51 | 433/– | 313/– | 270, 338 | apigenin 6-C-glucosidea |
O6 | 25.00 | 463/– | 343/– | 270, 338 | diosmetin 6-C-glucoside |
O7 | 25.51 | 595/593 | 287/285 | 256, 268, 350 | luteolin 7-O-glucoside |
O9 | 31.78 | 609/607 | 463, 301/299 | 252, 268, 348 | diosmetin 7-O-rutinosidea |
O10 | 30.68 | 609/607 | 463, 301/299 | 252, 268, 348 | diosmetin 7-O-neohesperidosidea |
glycosylated flavanones (group IV) | |||||
O3 | 17.26 | 743/741 | 581, 419, 273/271 | 286, 336 sh | naringenin triglycoside |
O4 | 22.28 | 773/771 | 611, 449, 303/301 | 286, 336 sh | hesperitin triglycoside |
O8 | 30.50 | 581/579 | 419, 273/271 | 284, 336 sh | naringenin 7-O-rutinosidea |
O11 | 33.87 | 611/609 | 449, 303/301 | 284, 336 sh | hesperidina |
O12 | 46.87 | 595/593 | 433, 287/285 | 284, 336 sh | didymina |
polymethoxyflavones (group 1) | |||||
O13 | 57.30 | 373/– | 268 sh, 330 | sinengetina | |
O14 | 59.41 | 403/– | 224, 334 | nobiletina | |
O15 | 60.56 | 403/– | 268, 302 | quercetigenin hexamethyl ether | |
O16 | 61.16 | 343/– | 272, 302 | tetramethylscutellarein | |
O17 | 62.62 | 433/– | 268 sh, 342 | heptamethoxyflavone | |
O18 | 64.33 | 373/– | 226, 330 | tangeretina |
Identification with standard.
Over half of the hydroxycinnamates were first detected in this plant.
At the same LC condition, the retention time of isoferulic acid is 27.42 min.