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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Arthritis Rheum. 2013 Apr;65(4):10.1002/art.37825. doi: 10.1002/art.37825

Figure 1.

Figure 1

Protein kinase Cβ (PKCβ) deficiency prevents lupus development in Sle mice. A, Levels of serum IgG anti–double-stranded DNA (anti-dsDNA) and antihistone/anti-dsDNA autoantibodies from the indicated mouse strains (8–10 months old), as determined by enzyme-linked immunosorbent assay. For analysis of anti-dsDNA IgG and antihistone/anti-dsDNA, sera were diluted 100-fold and 200-fold, respectively. Values are the mean ± SD of ≥3 mice per group. B, Frozen kidney sections from 5-month-old mice, stained with fluorescein isothiocyanate–labeled anti-IgG2b or anti-IgG2c. C, Paraffin-embedded kidney sections from the same mice as in B, stained with periodic acid–Schiff reagent. Enlarged glomeruli that are typical of Sle1.Sle3 mice were not observed in PKCβ-deficient Sle mice. Images in B and C are representative of 2 independent experiments. D, Proteinuria levels, as measured with Uristix strips. Values are the mean ± SD of 8–10 mice per strain. * = P <0.05; ** = P < 0.01. In B and C, original magnification × 20 and × 40, respectively.