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. 2013 Sep 4;8(9):e73969. doi: 10.1371/journal.pone.0073969

Figure 8. Expression of memory markers on CD4+ lymphocytes from different tissues.

Figure 8

Cryopreserved lymphocytes from GALT of colon and duodenum, and PBMC from an uninfected pigtailed macaque and a human subject were stained with panels of Abs (either CD4, CD45RO, CD95, CD28 or CD4, CD45RO, CCR7, CCR5). Lymphocytes from the colon and duodenum were also live/dead stained. Acquired cells were gated on singlets, live cells and then lymphocytes before gating on CD4 and CD45RO. CD45RO+ and CD45RO- subsets of CD4+ cells were analyzed for CD95 and CD28, or CCR7 and CCR5. Left: CD4 and CD45RO expression. Center: CD95 and CD28 expression on CD4+ CD45RO- and CD4+ CD45RO+ cells. Right: CCR7 and CCR5 expression of CD4+ CD45RO- and CD4+ CD45RO+ cells.