Table 1. Antibodies used in these studies.
Clone | Fluor | Source | |
---|---|---|---|
CD45RO | UCHL1 | unconjugated | Hybridoma |
FITC, PE, PerCP-Cy5.5,APC | eBioscience, BD, Icyt | ||
biotin | described in methods | ||
CD45RA | 5H9 | FITC | BD |
CD3 | SP34-2 | FITC,PerCP, APC-Cy7 | BD |
CD4 | CD4R1, L200, S3.5 | Alexa488, PerCP, Qdot605 | NIH, BD, Life Technologies |
CD8 | RPA-T8 | Pacific Blue | BD |
CD95 | DX2 | APC | eBioscience |
CD28 | CD28.2 | PerCP-Cy5.5 | eBioscience |
CCR5 | 3A9 | PE | BD |
CCR7 | 3D12 | APC | eBioscience |
CD2 | S5.2 | FITC | BD |
CD20 | L27 | PerCP | BD |
CD14 | M5E2 | APC | BD |
Mouse IgG | Polyclonal | Alexa488 | Invitrogen |
Ricin A chain | RAC18 | Alexa488 | [40] |