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. 2013 Sep 4;8(9):e73935. doi: 10.1371/journal.pone.0073935

Figure 1. Detailed experimental design.

Figure 1

A) DNA samples from ten species were pooled together at equimolar (T1), decreasing (T2), and increasing concentrations (T3); each treatment was then PCR-amplified using specific fusion primers. B) Three distinct PCR-amplifications were run for each species individually, using distinct fusion primers. PCR products with identical primer tags were then pooled together at varying concentrations (T4, T5, T6). C) One water and one sediment samples, were collected; each sample was divided into three sub-samples and spiked with either 1 larva (T7, T9), 5 larvae (T8, T10), or no larva (controls T11, T12) of the Northern-Pacific seastar Asterias amurensis (genus Asterias does not occur in New Zealand), and PCR-amplified. Treatments 1 to 6 and 7 to 12 were pooled together and analysed in multiplex on the 454 GS Junior™ pyrosequencer.