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. 2013 Sep 4;8(9):e74731. doi: 10.1371/journal.pone.0074731

Figure 3. Evaluation of transient gene silencing using semiquantitative-PCR.

Figure 3

Transcript expression of steviol glycoside pathway specific genes was analyzed on 5, 7, 9 and 12 days post infiltration (dpi). Untransformed wild was used as control. Transcript expression of 26S rRNA was used as internal control. Bar diagram shows the densitometric quantification of relative mRNA accumulation of (A) SrKA13H, (B) SrUGT85C2, (C) SrUGT74G1 and (D) SrUGT76G1 on agroinfiltration of gene specific RNAi constructs with respect to internal control. The RNAi constructs corresponding to these four genes were found to significantly reduce the transcript expression of respective endogenous genes.