Table 2. Binding energy (kCal/mol) of SrUGTs of steviol glycoside biosynthesis pathway with the substrates 19-O-β-glucopyranosyl steviol and rubusoside.

Protein/Substrate	19-O-β-glucopyranosyl steviol	Rubusoside
SrUGT85C2	–4.42	+4.08
SrUGT74G1	–5.15	+16.36
SrUGT76G1	–7.11	–4.79

Lowest the binding energy, highest will be the binding affinity.