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. 2013 Sep 4;8(9):e73525. doi: 10.1371/journal.pone.0073525

Figure 1. Expression of the sialidase and selected hyaluronidase genes in the wild-type, mutant and complemented strains.

Figure 1

RNA was isolated from cells grown in TPYG broth for 4 h, which corresponds to exponential growth phase. The relative expression of the nanI, nanJ, nagJ and nagL (A) and nagK (B) genes was determined by QRT-PCR. Expression levels are relative to the expression of the housekeeping gene rpoA and are the average of three independent biological replicates (± SEM). The asterisk (*) denotes a statistically significant difference (p≤0.05) as calculated using the student’s t-test.