|
BACTERIAL STRAINS
|
|
E. coli
|
| DH5α |
F−Ф80 lacZΔM15Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 (rk
− mk
−) deoR λ–
thi-1 phoA supE44 gyrA96 relA1
|
Life Technologies |
|
C. perfringens
|
| JIR325 |
RifRNalR derivative of strain 13 |
[14]
|
| JIR12192 |
JIR 325ΔreeSΩerm(Q) |
This Study |
| JIR12578 |
JIR12192(pJIR3818) |
This Study |
|
PLASMIDS
|
| pJIR418 |
E. coli-C. perfringens shuttle vector, carries pIP404 replication origin, CmR ErmR
|
[31]
|
| pJIR2715 |
Suicide vector base for C. perfringens, CmR ErmR
|
[30]
|
| pJIR3375 |
pJIR2715 (Asp718/BamHI)ΩJRP3524/JRP3526 (Asp718/BamH1, 1.5 kb) PCR product, CmR ErmR
|
This Study |
| pJIR3377 |
pJIR3377 (XbaI/NotI)ΩJRP3526/JRP3527 (XbaI/NotI, 1.5 kb) PCR product, CmR ErmR
|
This Study |
| pJIR3818 |
pJIR418 (8 kb EcoRI fragment containing reeS) CmR ErmR
|
This Study |
