Table 1. Validation of microarray data in fold changes using quantitative real-time RT-PCR.
|
HLSV vs Mock (12 dpi)
|
HLSV+TMVvs TMV(15 dpi)
|
||||
|---|---|---|---|---|---|
| Gene | Real time RT-PCR | Microarray | Real time RT-PCR | Microarray | Validated |
| NbAGO4-2 | 1.29 | 1.04 | 1.23 to 1.51 | -1.06 | Yes |
| NbARP1 | 1.03 | -1.17 to 1.14 | 2.51 | 1.84 to 2.06 | Yes |
| NbCaM3 | 1.49 | 2.33 to 2.97 | 2.06 | 2.54 to 4.57 | Yes |
| NbCP2 | 3.15 | 2.78 to 3.60 | 6.66 | 3.20 to 4.67 | Yes |
| NbPI | 1.64 | 2.26 to 2.31 | -3.19 | -9.14 to -9.29 | Yes |
| NbACO | 1.63 | 1.95 | -1.52 | -16.17 | Yes |
| NbALY | 1.26 | -1.06 to 1.01 | -1.19 to 1.17 | 1.06 to 1.13 | Yes |
| NbCAF1 | -1.50 to 1.64 | -1.05 to 12.61 | -1.21 | -1.02 to 11.35 | Yes |
| NbCAT1 | 1.65 | 2.19 | 1.95 | 3.01 | Yes |
| NbHSP101 | 1.35 | -2.34 | -1.33 | -17.14 | Yes |
| NbSAR8.2m | 1.21 | 1.72 to 1.80 | -18.00 | -6.90 to -6.86 | Yes |
| NbWIPK | 1.66 | -1.70 | -1.97 | -3.62 | Yes |
| NbWRKY7 | -1.39 | -1.09 | -5.89 | -1.06 | Yes |
| NbWRKY8 | -1.23 | -1.74 | -2.76 | -3.55 | Yes |
| NbTOM1 | -1.05 to 1.65 | 1.29 | 1.32 | 1.40 | Yes |
| NbVPE1α | 2.31 | 2.39 | 1.07 to 2.28 | 1.84 | Yes |