Figure 6. Mito-TEMPO ameliorates hyperthermia-impaired platelet function.

(A) Platelet aggregation was performed as described in Methods. Representative traces from three independent experiments are shown. (B and C) Washed platelets were incubated at the indicated temperatures for 3 h (B), or pre-incubated with Mito-TEMPO, GM6001 and solvent control at 37°C for 15 min, followed by incubation at 42°C for 3 h (C). Western blot was performed as described in Methods. (D and E) Washed platelets were incubated at indicated temperatures for 3 h (D), or pre-incubated without (control) or with Mito-TEMPO and solvent at 37°C for 15 min, and then incubated at 42°C for 3 h (E). Platelet adhesion was performed as described in Methods. The results shown are the mean ± SEM of cell number/mm². *P<0.017 (after Bonferroni correction) as compared with RT, **P<0.025 (after Bonferroni correction) as compared with control. Mito-TEMPO is labeled as TEMPO.