Figure 2. Treg frequency has negative correlation with severity of cardiac fibrosis.

Groups of 20 mice were infected with 10 TCID₅₀ dose of CVB3, 1,2,3,4 wks after infection, spleen and myocardial infiltrated cells were subjected to, flow cytometry protocol and stained for PerCP-CD4 and PE-Foxp3. (A) Frequencies of peripheral and myocardial Tregs were shown, graph representative of three independent experiments. (B) Relative numbers of Treg frequency. Data. represent as mean ±SEM. *, P<0.05 compared to non-infected mice (wk0). N=5 mice per group (C) Myocardial Treg frequency and the relative collagen volume fraction (CVF) of heart tissue were parallelly shown in one graph. (D and E) 4 weeks after CVB3 infection, peripheral Treg frequency and CVF or collagen I mRNA in each mouse were analyzed and correlated.