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. 2013 Sep 4;8(9):e73285. doi: 10.1371/journal.pone.0073285

Table 1. Protein Spot with Variable of importance (VIP) value >1.0 in the PLS-DA multivariate model.

ID (spotnr) Protein ID (SwissProt) Protein Mw/pI MS Score No. of peptides Av. Ratio (MYA/CON) VIP PLS-DA
Metabolic
269 P11217 Glycogen phosporylase, muscle form 97.5/6.6 134 4 1.47 1.9
462 P14618 Pyruvate kinase isoenzymes M1/M2 58.5/8.0 472 14 −1.16 1.7
640 P13929 Beta Enolase 46.8/7.7 1010 16 −1.11 1.6
804 P04075 Fructose bisphosphate adolase A&C 39.4/8.3 2795 14 −1.18 1.4
170 Q99798 Aconitate hydratase, mitochondrial 86.1/7.4 146 4 −1.40 1.3
310 Q02218 2-oxoglutarate dehydrogenase, mitochondrial 117/6.4 282 7 1.23 1.3
518 P13645 Creatine kinase, M-type 43/6.8 142 2 −1.16 1.3
546 P25705 ATP synthase, subunit alpha 55/8.3 129 4 −1.46 1.2
883 P04406 Glyceraldehyde-3-phosphate dehydrogenase 35.9/8.6 2062 14 −1.16 1.2
437 P36871 Phosphoglucomutase-1 61.7/6.3 797 19 1.07 1.1
Contractile
381 P60709 Actin, cytoplasmic 1 41.6/5.3 64 2 −1.24 1.8
41 P62736 Actin aortic smooth muscle 42.4/5.23 99 3 1.36 1.3
383 Q00872 Myosin binding protein C, slow type 129/5.8 273 10 1.18 1.3
448 P12883 Myosin-7, slow 223/5.6 207 6 1.25 1.3
1186 Q96A32 Myosin regulatory light chain 2, skeletal muscle isoform 19.01/4.9 93 13 1.83 1.2
162 P13533 Myosin-6, fast 223.7/5.6 140 8 −1.42 1.2
573 Q9UKX2 Myosin-2 224/5.6 638 23 −1.12 1.1
412 P12883 Myosin-7, slow 223/5.6 93 6 −1.52 1.0
Acute response
86 P08107 Heat shock 70 kDa protein 70.2/5.5 944 25 1.16 1.4
1051 P07451 Carbonic anhydrase 3 29.4/6.9 146 13 −1.32 1.5
422 P01009 Alpha-1-antitrypsin 46.7/5.4 99 2 1.31 1.2
Structural
849 P04264 Keratin, type II cytoskeletal 1 66/8.1 382 13 −1.28 1.7
259 O75112 LIM domain-binding protein 3 77/8.5 155 2 −1.40 1.2
525 P07437 Tubulin beta chain 50/4.8 787 16 −1.17 1.2
428 P40123 Adenylyl cyclase associated protein 2 53/5.9 135 4 1.20 1.1
490 Q8TD99 Desmin 53.5/5.2 1059 39 1.28 1.1
Other
179 P02768 Serum albumin 66.5/5.7 381 13 −1.13 1.4
807 P82650 28S ribosomal protein S22, mitochondrial 41/7.7 130 4 −1.27 1.3

Proteins identified with LC-MS/MS, MALDI-TOF-MS/MS. ID is the same as in figure 2 (Weight-plot PLS-DA).