Table 1. Protein Spot with Variable of importance (VIP) value >1.0 in the PLS-DA multivariate model.
ID (spotnr) | Protein ID (SwissProt) | Protein | Mw/pI | MS Score | No. of peptides | Av. Ratio (MYA/CON) | VIP PLS-DA |
Metabolic | |||||||
269 | P11217 | Glycogen phosporylase, muscle form | 97.5/6.6 | 134 | 4 | 1.47 | 1.9 |
462 | P14618 | Pyruvate kinase isoenzymes M1/M2 | 58.5/8.0 | 472 | 14 | −1.16 | 1.7 |
640 | P13929 | Beta Enolase | 46.8/7.7 | 1010 | 16 | −1.11 | 1.6 |
804 | P04075 | Fructose bisphosphate adolase A&C | 39.4/8.3 | 2795 | 14 | −1.18 | 1.4 |
170 | Q99798 | Aconitate hydratase, mitochondrial | 86.1/7.4 | 146 | 4 | −1.40 | 1.3 |
310 | Q02218 | 2-oxoglutarate dehydrogenase, mitochondrial | 117/6.4 | 282 | 7 | 1.23 | 1.3 |
518 | P13645 | Creatine kinase, M-type | 43/6.8 | 142 | 2 | −1.16 | 1.3 |
546 | P25705 | ATP synthase, subunit alpha | 55/8.3 | 129 | 4 | −1.46 | 1.2 |
883 | P04406 | Glyceraldehyde-3-phosphate dehydrogenase | 35.9/8.6 | 2062 | 14 | −1.16 | 1.2 |
437 | P36871 | Phosphoglucomutase-1 | 61.7/6.3 | 797 | 19 | 1.07 | 1.1 |
Contractile | |||||||
381 | P60709 | Actin, cytoplasmic 1 | 41.6/5.3 | 64 | 2 | −1.24 | 1.8 |
41 | P62736 | Actin aortic smooth muscle | 42.4/5.23 | 99 | 3 | 1.36 | 1.3 |
383 | Q00872 | Myosin binding protein C, slow type | 129/5.8 | 273 | 10 | 1.18 | 1.3 |
448 | P12883 | Myosin-7, slow | 223/5.6 | 207 | 6 | 1.25 | 1.3 |
1186 | Q96A32 | Myosin regulatory light chain 2, skeletal muscle isoform | 19.01/4.9 | 93 | 13 | 1.83 | 1.2 |
162 | P13533 | Myosin-6, fast | 223.7/5.6 | 140 | 8 | −1.42 | 1.2 |
573 | Q9UKX2 | Myosin-2 | 224/5.6 | 638 | 23 | −1.12 | 1.1 |
412 | P12883 | Myosin-7, slow | 223/5.6 | 93 | 6 | −1.52 | 1.0 |
Acute response | |||||||
86 | P08107 | Heat shock 70 kDa protein | 70.2/5.5 | 944 | 25 | 1.16 | 1.4 |
1051 | P07451 | Carbonic anhydrase 3 | 29.4/6.9 | 146 | 13 | −1.32 | 1.5 |
422 | P01009 | Alpha-1-antitrypsin | 46.7/5.4 | 99 | 2 | 1.31 | 1.2 |
Structural | |||||||
849 | P04264 | Keratin, type II cytoskeletal 1 | 66/8.1 | 382 | 13 | −1.28 | 1.7 |
259 | O75112 | LIM domain-binding protein 3 | 77/8.5 | 155 | 2 | −1.40 | 1.2 |
525 | P07437 | Tubulin beta chain | 50/4.8 | 787 | 16 | −1.17 | 1.2 |
428 | P40123 | Adenylyl cyclase associated protein 2 | 53/5.9 | 135 | 4 | 1.20 | 1.1 |
490 | Q8TD99 | Desmin | 53.5/5.2 | 1059 | 39 | 1.28 | 1.1 |
Other | |||||||
179 | P02768 | Serum albumin | 66.5/5.7 | 381 | 13 | −1.13 | 1.4 |
807 | P82650 | 28S ribosomal protein S22, mitochondrial | 41/7.7 | 130 | 4 | −1.27 | 1.3 |
Proteins identified with LC-MS/MS, MALDI-TOF-MS/MS. ID is the same as in figure 2 (Weight-plot PLS-DA).