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. 2013 Sep 4;8(9):e73542. doi: 10.1371/journal.pone.0073542

Figure 5. Identification of the repressive p53 RE in GFI1 core promoter.

Figure 5

(A) Nucleotide sequences of wild type (WT) and mutated GFI1 core promoter fragments as compared with conserved repressive p53 RE. Potential p53 RE in GFI1 core promoter are underlined. Mutated nucleotides are in bold. (B) p53 −/− HCT116 cells were transfected with WT or M1 GFI1 promoter (−1933/+468 bp; left panel), or WT or M2 GFI1 promoter (−460/+6 bp; right panel) luciferase reporter construct without or with p53. Luciferase activity was measured 36 hrs after transfection. (C) p53 −/− HCT116 cells were transfected with pGL3-basic plasmid containing WT or M1 GFI1 promoter fragment (−4840/+184 bp) along with p53. Binding of p53 to the GFI1 promoter fragments was examined by ChIP assays (left panel). Expression of p53 was confirmed by Western blot analysis (right panels).