Figure 7. Overexpression of Gfi1 inhibits DNA damage-induced cell death.

Ba/F3 (A–C) and Ramos (D–F) cells were transduced with the inducible lentiviral expression construct for Gfi1 and examined for Gfi1 expression by Western blot analysis after incubation with Doxy (1 µg/ml) for 24 hrs (A and D). Cells were then exposed to Doxo (100 ng/ml for Ba/F3 cells and 2 mg/ml for Ramos cells) for 18 hrs with or without preincubation with Doxy (1 µg/ml) for 24 hrs. Cell viabilities were determined by exclusion of trypan blue staining (B and E), percentages of apoptotic (annexin V-positive) cells were assessed by flow cytometry after staining with annexin V and 7-AAD (C), and the numbers of living cells were quantitated by MTS assay (F).