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. 2013 Sep 4;8(9):e72530. doi: 10.1371/journal.pone.0072530

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© 2013 Steglich et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Serum samples from immunized chickens before challenge collected at day 20 (MDA-) or 21 (MDA+) after vaccination with chNDVFHN _PMV8H5 were tested for NDV (A), APMV-8 (B) and AIV H5 (C) specific antibodies by HI test. Furthermore, serum samples of MDA- chickens and MDA+ chickens vaccinated at day one (MDA+a) or day 7 (MDA+b) after hatch, taken 14 dpch were tested for AIV H5 (D) specific antibodies by HI assay. The number of animals considered seropositive (HI titer > 2 log₂) is given below box plots. Significant differences (P < 0.05) between C and D are marked by *.