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. 2013 Sep 4;8(9):e74342. doi: 10.1371/journal.pone.0074342

Figure 1. CX-4945 inhibits TGF-β1-induced EMT.

Figure 1

(A) The chemical structure of CX-4945. (B) The effect of CX-4945 on the viability of A549 cells in the presence of TGF-β1. Briefly, cells wre treated with TGF-β1 (5 ng/ml) alone or in combination with CX-4945 for 72 h, and then cell viability was measured. **, p < 0.01 (versus the control). The effect of CX-4945 on the TGF-β1-induced expression of epithelial and mesenchymal markers was evaluated by Western blot analysis (C) and immunocytochemistry (D). Briefly, after 24 h serum starvation, A549 cells were treated with TGF-β1 (5 ng/ml) alone or in combination with CX-4945 in media containing 0.1% FBS for 72 h. All scale bars represent 200 µm.