Skip to main content
. 2013 Sep 4;8(9):e72527. doi: 10.1371/journal.pone.0072527

Figure 1. Modulation of cps and flhDC operons by the RcsC- or RcsD-dependent presence.

Figure 1

The β-galactosidase activity (Miller units), produced by rcsB overexpression, from cps::MudJ (A) and flhDC::MudJ (B) lac transcriptional fusions was investigated in the following genetic backgrounds: wild-type, rcsD, rcsC and rcsD rcsC, harboring the prcsB (black bars) or prcsBop (grey bars) plasmids. These strains were grown at 37°C in LB medium supplemented with 0.35 mM IPTG. The osmotic shock effect by sucrose addition (striped bars) on cps::MudJ (C) and flhDC::MudJ (D) expression was investigated in the wild-type, rcsD, rcsC and rcsD rcsC strains; and compared with control without sucrose (empty bars). These assays were performed as described in Material and Methods. The error bars correspond to the standard deviation of three independent experiments done in duplicate.