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The RcsCcyt-His6 (A) or RcsDcyt-His6 (B) proteins were incubated with [γ-32P] ATP as described in Material and Methods. The phosphorylation was stopped at different time points (0 to 30 min) by addition of 4× SDS sample buffer, and subjected to electrophoresis analysis in a 12% SDS-PAGE and them exposed to autoradiography.
