Table 1. Bacterial strains and plasmids used in this study.
Strain or plasmid | Descriptiona | Reference or source |
S. Typhimurium strains | ||
14028s | wild-type | Fields et al. (1986) [37] |
EG13384 | cps::MudJ | Mouslim and Groisman (2003) [38] |
EG13772 | flhDC5213::MudJ | Mouslim et al. (2003) [39] |
EG14498 | ΔrcsC::Cm | Pescaretti et al. (2009) |
MDs1117 | ΔrcsD::Cm | This work |
MDs1574 | cps::MudJ ΔrcsD::Cm | This work |
MDs1575 | cps::MudJ ΔrcsC::Cm | This work |
MDs1576 | cps::MudJ ΔrcsD ΔrcsC::Cm | This work |
MDs1577 | flhDC5213::MudJ ΔrcsD::Cm | This work |
MDs1578 | flhDC5213::MudJ ΔrcsC::Cm | This work |
MDs1579 | flhDC5213::MudJ ΔrcsD ΔrcsC::Cm | This work |
MDs1557 | cps::MudJ ackA::Tn10Tc | This work |
MDs1558 | cps::MudJ ackA::Tn10Tc ΔrcsC::Cm | This work |
MDs1559 | cps::MudJ ackA::Tn10Tc ΔrcsD::Cm | This work |
MDs1562 | cps::MudJ ackA::Tn10Tc ΔrcsD::FRT ΔrcsC::Cm | This work |
E. coli strains | ||
DHM1 | F-, cya-854, recA1, endA1, gyrA96 (Nal), thi1, hsdR17,spoT1, rfbD1, glnV44(AS). | Karimova et al. (2005) |
MDs1265 | BL21 DE3 ΔrcsDBC::Cm | This work |
Plasmids | ||
pUHE2-2lacIq | reppMB1 Apr lacIq | Soncini et al. (1995) [40] |
prcsB | pUHE2-21 lacIq containing rcsB gene | Pescaretti et al. (2009) |
prcsBop | pUHE2-21 lacIq containing rcsB gene in opposite direction of the lac promoter | Pescaretti et al. (2009) |
pT7-7 | repPMB1 Apr pT7 | Tabor and Richardson (1985) |
pT7-7-rcsB-His6 | repPMB1 Apr pT7-rcsB-His6 | Delgado et al. (2006) |
pTrcsCcytHT | repPMB1 Apr pT7-rcsCcyt-His6 | This work |
pTrcsDcytHT | repPMB1 Apr pT7-rcsDcyt-His6 | This work |
pUT18 | Apr, Col E1 ori, vector for fusion to N-terminus of Cya-T18 | Karimova et al. (1998) |
pUT18C | Apr, Col E1 ori, vector for fusion to C-terminus of Cya-T18 | Karimova et al. (1998) |
pKT25 | Kmr, p15A ori, vector for fusion to C-terminus of Cya-T25 | Karimova et al. (1998) |
pKTN25 | Kmr, p15A ori, vector for fusion to N-terminus of Cya-T25 | Karimova et al. (1998) |
pUT18C-Zip | pUT18 with the leucine zipper domain of the yeast GCN4 activator | Karimova et al. (1998) |
pKT25-Zip | pKT25 containing leucine zipper domain of the yeast GCN4 activator | Karimova et al. (1998) |
pKD3 | bla FRT cat FRT PS1 PS2 oriR6K | Datsenko and Wanner (2000) |
pKD46 | bla PBAD gam bet exo pSC101 oriTS | Datsenko and Wanner (2000) |
Gene designations are summarized by Sanderson et al. (1995) [41]. Nal, nalidixic acid resistance; Ap, ampicillin resistance; Km, kanamycin resistance; Cm, Cloramphenicol resistance.