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. 2013 Sep 4;8(9):e72527. doi: 10.1371/journal.pone.0072527

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Descriptiona Reference or source
S. Typhimurium strains
14028s wild-type Fields et al. (1986) [37]
EG13384 cps::MudJ Mouslim and Groisman (2003) [38]
EG13772 flhDC5213::MudJ Mouslim et al. (2003) [39]
EG14498 ΔrcsC::Cm Pescaretti et al. (2009)
MDs1117 ΔrcsD::Cm This work
MDs1574 cps::MudJ ΔrcsD::Cm This work
MDs1575 cps::MudJ ΔrcsC::Cm This work
MDs1576 cps::MudJ ΔrcsD ΔrcsC::Cm This work
MDs1577 flhDC5213::MudJ ΔrcsD::Cm This work
MDs1578 flhDC5213::MudJ ΔrcsC::Cm This work
MDs1579 flhDC5213::MudJ ΔrcsD ΔrcsC::Cm This work
MDs1557 cps::MudJ ackA::Tn10Tc This work
MDs1558 cps::MudJ ackA::Tn10Tc ΔrcsC::Cm This work
MDs1559 cps::MudJ ackA::Tn10Tc ΔrcsD::Cm This work
MDs1562 cps::MudJ ackA::Tn10Tc ΔrcsD::FRT ΔrcsC::Cm This work
E. coli strains
DHM1 F-, cya-854, recA1, endA1, gyrA96 (Nal), thi1, hsdR17,spoT1, rfbD1, glnV44(AS). Karimova et al. (2005)
MDs1265 BL21 DE3 ΔrcsDBC::Cm This work
Plasmids
pUHE2-2lacIq reppMB1 Apr lacIq Soncini et al. (1995) [40]
prcsB pUHE2-21 lacIq containing rcsB gene Pescaretti et al. (2009)
prcsBop pUHE2-21 lacIq containing rcsB gene in opposite direction of the lac promoter Pescaretti et al. (2009)
pT7-7 repPMB1 Apr pT7 Tabor and Richardson (1985)
pT7-7-rcsB-His6 repPMB1 Apr pT7-rcsB-His6 Delgado et al. (2006)
pTrcsCcytHT repPMB1 Apr pT7-rcsCcyt-His6 This work
pTrcsDcytHT repPMB1 Apr pT7-rcsDcyt-His6 This work
pUT18 Apr, Col E1 ori, vector for fusion to N-terminus of Cya-T18 Karimova et al. (1998)
pUT18C Apr, Col E1 ori, vector for fusion to C-terminus of Cya-T18 Karimova et al. (1998)
pKT25 Kmr, p15A ori, vector for fusion to C-terminus of Cya-T25 Karimova et al. (1998)
pKTN25 Kmr, p15A ori, vector for fusion to N-terminus of Cya-T25 Karimova et al. (1998)
pUT18C-Zip pUT18 with the leucine zipper domain of the yeast GCN4 activator Karimova et al. (1998)
pKT25-Zip pKT25 containing leucine zipper domain of the yeast GCN4 activator Karimova et al. (1998)
pKD3 bla FRT cat FRT PS1 PS2 oriR6K Datsenko and Wanner (2000)
pKD46 bla PBAD gam bet exo pSC101 oriTS Datsenko and Wanner (2000)

Gene designations are summarized by Sanderson et al. (1995) [41]. Nal, nalidixic acid resistance; Ap, ampicillin resistance; Km, kanamycin resistance; Cm, Cloramphenicol resistance.