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. 2013 Sep 4;8(9):e74444. doi: 10.1371/journal.pone.0074444

Figure 4. Phosphate starvation promotes C4-HSL production.

Figure 4

PAO1, ΔpstS, ΔphoB and ΔpstSΔphoB were grown for 48 hours at 37°C with shaking in M9 medium. C4-HSL in each strain was extracted and measured as described in Materials and Methods. β-galactosidade activity (Miller Units) represent C4-HSL levels. Results represent mean+standard deviation of three different experiments. Each experiment was performed in triplicate. Asterisks represent the significant elevation in C4-HSL production in ΔpstS as opposed to all other strains (p<0.05, Turkey’s post hoc test).