Table 3. Ranking of candidate reference genes by three different algorithms.
Gene name | Ranking order | ||||||||||||
geNorm | NormFinder | BestKeeper | |||||||||||
Combined | WT | M | No group | 2-group | Combined | WT | M | ||||||
rsh | 1/2 | 1/2 | 1/2 | 1 | 1 | 1 | 2 | 1 | |||||
rpoD | 1/2 | 1/2 | 3 | 2 | 3 | 2 | 1 | 3 | |||||
gltA | 3 | 3 | 1/2 | 4/5 | 2 | 3 | 4 | 2 | |||||
GAPDH | 4 | 4 | 4 | 3 | 4 | 4 | 3 | 4 | |||||
rpsL | 5 | 5 | 5 | 4/5 | 5 | 5 | 5 | 5 | |||||
16S rRNA | 6 | 6 | 6 | 6 | 6 |
GeNorm (75), NormFinder (2) and BestKeeper (51) used to identify the most stably expressed genes in control and HL conditions. Wild type (WT) and mutant (M) strains of Planktothrix agardhii.