Table 2. Candidate reference genes for Biotic Stress effect in V. vinifera cv Trincadeira calculated by the GeNorm, NormFinder and BestKeeper.
Candidate genes | Trincadeira | |||
GeNorm | NormFinder | BestKeeper | ||
M | SV | SD | r | |
SAND | 0.881 (1/2) | 0.727 (3) | 0.92 (5) | 0.74* |
EF1α | 0.881(1/2) | 0.722 (2) | 0.86 (3) | 0.87* |
SMD3 | 0.975 (3) | 0.454 (1) | 0.66 (1) | 0.76* |
ACT | 1.038 (4) | 0.849 (5) | 0.92 (4) | 0.75* |
UBQ | 1.088 (5) | 1.231 (6) | 1.00 (6) | 0.58* |
GAPDH | 1.158 (6) | 0.813 (4) | 0.84 (2) | 0.81* |
60 S | 1.305 (7) | 1.511 (7) | 1.09 (8) | 0.25* |
PsaB | 1.470 (8) | 1.799 (8) | 1.41 (7) | 0.74* |
SV. stability value; SD, standard deviation of Cq value; r. Pearson coefficient of correlation; * p≤0.001. p-value associated with the Pearson coefficient of correlation. Ranking order is presented in parenthesis.