Table 4. Multivariate a Predictors of Cord and Three Year DNA Methylation.
| Cord DNA methylation (N = 237) (ng/100ng total DNA) | Cord DNA methylation (subset) (n = 139)b (ng/100ng total DNA) | Three year DNA methylation (n = 139) (ng/100ng total DNA) | Three year DNA methylation, adj for cord methylation (N = 139) (ng/100ng total DNA) | |||||||||
| Beta | 95% CI | Beta | 95% CI | Beta | 95% CI | Beta | 95% CI | |||||
| Maternal Education | ||||||||||||
| HS (vs. no HS) | 0.103 | −0.291, | 0.496 | 0.301 | −0.196, | 0.798 | 0.033 | −0.412, | 0.478 | −0.005 | −0.448, | 0.439 |
| >HS (vs. no HS) | 0.090 | −0.328, | 0.509 | 0.077 | −0.498, | 0.652 | −0.281 | −0.795, | 0.233 | −0.299 | −0.809, | 0.211 |
| Married (vs. unmarried) | −0.190 | −0.604, | 0.224 | −0.275 | −0.836, | 0.287 | 0.135 | −0.368, | 0.638 | 0.163 | −0.336, | 0.663 |
| Material Hardship | 0.094 | −0.234, | 0.421 | 0.091 | −0.349, | 0.530 | −0.191 | −0.584, | 0.202 | −0.199 | −0.589, | 0.191 |
| Afr. Am. (vs. Dominican) | −0.030 | −0.395, | 0.334 | 0.052 | −0.473, | 0.576 | −0.052 | −0.532, | 0.427 | −0.073 | −0.549, | 0.402 |
| Prenatal ETS | −0.068 | −0.435, | 0.298 | 0.046 | −0.458, | 0.549 | 0.247 | −0.239, | 0.733 | 0.215 | −0.268, | 0.698 |
| Former smoker | 0.010 | −0.355, | 0.374 | −0.304 | −0.829, | 0.220 | −0.470 | −0.949, | 0.009 | −0.437 | −0.913, | 0.038 |
| Male (vs. female) | −0.036 | −0.356, | 0.284 | −0.280 | −0.708, | 0.148 | −0.265 | −0.650, | 0.120 | −0.227 | −0.611, | 0.158 |
| Multiparous (vs. nulliparous) | −0.060 | −0.435, | 0.314 | −0.020 | −0.543, | 0.503 | 0.099 | −0.365, | 0.564 | 0.107 | −0.354, | 0.568 |
| Pre-pregnancy BMI | −0.035 | −0.062, | −0.007 | −0.044 | −0.081, | −0.008 | −0.069 | −0.102, | −0.036 | −0.062 | −0.096, | −0.029 |
| Weight Gain | 0.000 | −0.010, | 0.011 | 0.003 | −0.012, | 0.018 | −0.010 | −0.023, | 0.004 | −0.010 | −0.023, | 0.004 |
| Maternal Age | −0.011 | −0.053, | 0.031 | −0.032 | −0.087, | 0.024 | −0.044 | −0.094, | 0.006 | −0.038 | −0.088, | 0.013 |
| Total PAH (log-adj) | 0.085 | −0.113, | 0.282 | 0.070 | −0.231, | 0.370 | 0.137 | −0.133, | 0.407 | 0.124 | −0.145, | 0.392 |
| Breast Fed | 0.008 | −0.012, | 0.027 | 0.006 | −0.014, | 0.025 | ||||||
| ETS at 3 yrs | −0.551 | −1.072, | −0.030 | −0.470 | −0.994, | 0.055 | ||||||
| Cord DNA methylation | 0.146 | −0.018, | 0.309 | |||||||||
| R2 | 0.0979 | 0.274 | 0.304 | 0.322 | ||||||||
| Adjusted R2 | 0.0363 | 0.188 | 0.207 | 0.221 | ||||||||
Multivariatea Predictors of Cord and Three Year DNA Methylation.
a
All multivariate models corrected for inter-plate variation.
b
The ‘subset’ includes children who have contributed both cord and three year DNA samples.
Abbreviations: adj (adjusted); HS (high school); Afr. Am. (African-American); ETS (Environmental Tobacco Smoke); BMI (Body Mass Index); PAH (Polycyclic Aromatic Hydrocarbons).