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Purification of azoreductase from Bacillus strain SF
| Purification step | Amt of protein (mg) | Activity (nkat) | Sp act (nkat/mg) | Purification factor (fold) | Yield (%) |
|---|---|---|---|---|---|
| Extract | 493.1 | 9,842 | 20 | 1.0 | 100.0 |
| (NH4)2SO4 precipitation | 193.2 | 8,104 | 42 | 2.1 | 82.3 |
| Anion-exchange chroma- tography | 2.1 | 1,767 | 850 | 42.6 | 18.0 |
| Affinity chromatography | 0.04 | 198 | 4,972 | 249.1 | 2.0 |
