Fig. 8. Michael-type addition reaction for hydrogel formation. (A) Schematic of hydrogel formation using the Michael-type addition reaction between vinyl sulfone groups of 4-arm PEG and cysteine residues present on the RLP. (B) Human aortic adventitial fibroblasts (AoAFs) were encapsulated during hydrogel formation and cell viability was evaluated via live/dead staining (fluorescent laser scanning confocal microscopy). AoAFs remained viable throughout the experiment, adopting a spread morphology (scale bar, 200 μm). Image reprinted from McGann et al. 250 with permission from John Wiley and Sons publishing. Copyright (2013).
