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. 2013 Sep 4;8(9):e72273. doi: 10.1371/journal.pone.0072273

Figure 2. Intracellular production of cyclic DnaA1-86.

Figure 2

Expression of IntC::DnaA1−86::IntN was induced by addition of 2 mM IPTG to cells growing exponentially at 30°C or by plating exponential growing cells on plates containing 2 mM IPTG followed by incubation at 30C. A. Intein-DnaA precursor and splice products were visualized by Western blot using polyclonal anti-DnaA antibodies. B. Growth on LB plates containing 2 mM IPTG (top) and phase-contrast images (bottom) of expressing IntC::IntN (left) or after three hours expression of IntC::DnaA1−86::IntN (right).