Table 3. Protein pairs that can be targeted by the R-BTH system.
| Interactiona | Growth on 5-FOA platesb | |
| DnaN-HolA | + | + |
| DnaN-HolB | ++ | + |
| DnaN-DnaN | +++ | − |
| DnaN-DnaX | ++ | + |
| DnaN-PolC | ++ | − |
| DnaX-HolB | ++ | + |
| DnaX-DnaX | ++ | − |
| DnaX-PolC | ++ | − |
| DnaA-DnaA | + | + |
| DnaB-DnaB | ++ | − |
| PolC-PolC | ++ | + |
| HolB-HolA | + | + |
| HolB-HolB | + | + |
| Vectors | − | + |
| Zip-Zip | +++ | − |
a
Strength of protein-protein interaction was obtained as in Table 2.
b
Was determined by plating on LB plates containing 1.3 μg/ml of 5-FOA. – indicates no growth, whereas + indicates appearance of colonies after 2 days incubation at 30°C.
Interacting pairs of proteins that did not promote growth on 5-FOA plates can be used in a selection for inhibitory compounds.