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. Author manuscript; available in PMC: 2013 Dec 16.
Published in final edited form as: Free Radic Biol Med. 2012 Oct 22;53(12):10.1016/j.freeradbiomed.2012.10.536. doi: 10.1016/j.freeradbiomed.2012.10.536

Fig. 5.

Fig. 5

Lack of Nrf2 increases oxidative stress. (A) To assess whether lack of Nrf2 leads to increased oxidative stress, the level of reactive oxygen species was measured in bone marrow stromal cells from Nrf2+/+ and Nrf2−/− mice by detecting oxidation of C400. (B) Total GSH level was measured in bone marrow stromal cells at 1 and 19 h postradiation time points (see Materials and Methods for details). (C) Representative immunoblot showing the expression of HO1 and catalase from Nrf2+/+ and Nrf2−/− mouse calverial osteoblasts with or without radiation exposure (24 h post-IR). Samples were collected 24 h after radiation exposure ex vivo. (D) Densitometric analysis of the relative expression of HO1 and catalase using ImageJ. (E) Calvarial osteoblasts from Nrf2+/+ and Nrf2−/− mice were exposed to 20 Gy single-dose radiation, NAC treatment, or a combination of both or were left untreated. Representative images of mineralized matrix formation under each condition are shown. (F) Quantification of VonKossa staining images from at least three different fields from two representative experiments using Metamorph software.