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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Biochem Pharmacol. 2013 Aug 5;86(6):770–781. doi: 10.1016/j.bcp.2013.07.024

FIGURE 3.

FIGURE 3

Proximal TCR signaling through ZAP-70 and LAT is impaired by treatment with PF-562,271. A) Western Blot analysis of total phospho-tyrosine levels relative to tubulin levels in human peripheral blood T cells in the presence or absence of 10 μM test compounds. Blots are representative of three independent experiments. B) Western Blot analysis of ZAP-70 at Y319 and LAT phosphorylation at Y191 relative to total protein levels in human peripheral blood T cells. Blots are representative of three independent experiments. C) Western Blot analysis of ERK and D) p38 MAPK phosphorylation relative to total protein levels in human peripheral blood T cells. Blots are representative of three independent experiments. E) Quantification of ZAP-70, LAT, and ERK phosphorylation (mean +/− SD, n=3, p < 0.05 relative to DMSO control (*) or relative to TCR control (#), ANOVA).