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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Addict Biol. 2013 May 27;19(1):10.1111/adb.12061. doi: 10.1111/adb.12061

Figure 6. Cocaine self-administration followed by 3 weeks of abstinence reduces surface expression of mGluR5 receptors in the dlSTR.

Figure 6

(A) The rat brain atlas coordinates according to Paxinos and Watson (2007) used to conduct tissue dissections. Representative immunoblot analysis of biotinylated slices isolated from the dlSTR. (B) The ratio of protein density in the surface (biotinylated) fraction to that in the total fraction was analyzed. We found a significant decrease in surface mGluR5s in cocaine relative to sucrose animals but no change in mGluR1 or CB1 receptor surface expression. (C) In the total fraction we found no differences in expression of mGluR5-associated proteins Homer 1b/c (H1b/c), calmodulin (CaM), or protein kinase C isoform ε (PKCε). * = p<0.05 in comparison to sucrose. N= 6–8/group. T - total lysate, I - intracellular fraction, S - surface fraction.