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. Author manuscript; available in PMC: 2014 Aug 12.
Published in final edited form as: Dev Cell. 2013 Aug 12;26(3):266–278. doi: 10.1016/j.devcel.2013.07.007

Figure 1. Exo70 induces tubular invaginations on synthetic vesicles.

Figure 1

(A) Transmission EM showing that GST-Exo70 induced tubular invaginations towards the interior of the lumen of LUVs similar to the MIM I-BAR domain. GST, GST-Exo70(K571A/E572A) did not induce any membrane tubules. See EXPERIMENTAL PROCEDURES for details. Scale bar, 0.5 μm. (B) Untagged Exo70 induced membrane invaginations. The arrow shows a representative region where the tubular invagination was connected to the exterior. See also Supplemental Movie 1a for 3-D tomography of this LUV. (C) Comparison of the diameters of the membrane tubules induced by different proteins. Error bars represent standard deviation (SD). n=80; *, p<0.01. (D) Confocal microscopy of fluorescence-labeled Giant Unilamellar Vesicles (GUVs) incubated with Exo70 (upper panel) or buffer control (lower panel). Sequential frames show the inward growth of a tubule (arrowhead) from a GUV incubated with Exo70. Scale bar, 5 μm.