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. 2013 Jul 5;305(6):L409–L418. doi: 10.1152/ajplung.00032.2013

Fig. 1.

Fig. 1.

Alveolar epithelial type II (ATII) cells spontaneously undergo transdifferentiation to alveolar epithelial type I (ATI) cells during culture. Freshly isolated mouse primary ATII cells were analyzed immediately (D0) or cultured on tissue culture plastic for one (D1), three (D3), five (D5), or seven (D7) days. A: the mRNA expression levels of ATII markers surfactant protein (Sftp) a, Sftpb, and Sftpc were measured by quantitative real-time PCR (qRT-PCR) and graphed; n = 3 experiments; *P < 0.05 compared with D0. B: the mRNA expression levels of ATI markers T1α and aquaporin 5 (Aqp5) were measured by qRT-PCR and graphed; n = 3; *P < 0.05 compared with D0. C: cell lysates were harvested at the stated times and immunoblotted for proSFTPC and T1α, with β-actin used as a loading control. Images are representative of 3 experiments with similar findings.