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. 2013 Jul 5;305(6):L409–L418. doi: 10.1152/ajplung.00032.2013

Fig. 8.

Fig. 8.

TGF-β and BMP signaling affects basal expression of ATII and ATI genes in MLE15 cells. MLE15 cells were transfected with small-interfering RNAs (siRNAs) targeting TGF-βR1, BMPR1a, and BMPR1b mRNAs or a nontargeting control. A: the mRNA expression of TGF-βR1, BMPR1a, BMPR1b was determined by qRT-PCR and graphed relative to values obtained with cells transfected with nontargeting oligonucleotides; n = 3; *P < 0.05. B: the mRNA expression of Sftpa, Sftpb, and Sftpc was determined by qRT-PCR and graphed relative to values obtained with cells transfected with nontargeting oligonucleotides; n = 3; *P < 0.05. C: the mRNA expression of T1α and Aqp5 was determined by qRT-PCR and graphed relative to values obtained with cells transfected with nontargeting oligonucleotides; n = 3; *P < 0.05. D: cell lysates were harvested and immunoblotted for proSFTPC and T1α, with β-actin used as a loading control.