Table 1.

Sequences of primers used in the quantitative real-time PCR

Genes	Sequences of Primers	GenBank Accession No.	Product Sizes, bp
Sftpa	F: 5′ TTCCAGGGTTTCCAGCTTACCT 3′	NM_023134.4	156
	R: 5′ AGTTGACTGACTGCCCATTGGT 3′
Sftpb	F: 5′ TGGAACACCAGTGAACAGGCTA 3′	NM_147779.1	103
	R: 5′ GCATGTGCTGTTCCACAAACTG 3′
Sftpc	F: 5′ TGATGGAGAGTCCACCGGATTA 3′	NM_011359.2	130
	R: 5′ CCTACAATCACCACGACAACGA 3′
T1α	F: 5′ AGCAAAGCCAAGACAGTATCGC 3′	NM_010329.2	179
	R: 5′ TTAGGACTGGGCTGGAATGTGT 3′
Aqp5	F: 5′ AGATCTCCATAGCCTTTGGCCT 3′	NM_009701.4	124
	R: 5′ AGCAGAGAGATCTGGTTGCCTA 3′
Tgf-β1	F: 5′ TACGTCAGACATTCGGGAAGCA 3′	NM_011577.1	138
	R: 5′ AGGTAACGCCAGGAATTGTTGC 3′
Tgf-β2	F: 5′ GCCTTCGCCCTCTTTACATTGA 3′	NM_009367.3	174
	R: 5′ CGGAAGCTTCGGGATTTATGGT 3′
Tgf-β3	F: 5′ CCTGGCCCTGCTGAACTTG 3′	NM_009368.3	75
	R: 5′ TTGATGTGGCCGAAGTCCAAC 3′
BMP-2	F: 5′ CACACAGGGACACACCAACC 3′	NM_007553.2	99
	R: 5′ CAAAGACCTGCTAATCCTCAC 3′
BMP-4	F: 5′ CGTCATTCCGGATTACATGAGGGA 3′	NM_007554.2	165
	R: 5′ CCTGGGATGTTCTCCAGATGTTCT 3′
Smad1	F: 5′ ACCCCTACCACTATAAGCGAG 3′	NM_008539.3	166
	R: 5′ TGCTGGAAAGAGTCTGGGAAC 3′
Smad2	F: 5′ ATGTCGTCCATCTTGCCATTC 3′	NM_010754.4	173
	R: 5′ AACCGTCCTGTTTTCTTTAGCTT 3′
Smad3	F: 5′ CACAGCCACCATGAATTACGG 3′	NM_016769.4	108
	R: 5′ TGGCGTCTCTACTCTCTGATAGT 3′
Smad5	F: 5′ TTGTTCAGAGTAGGAACTGCAAC 3′	NM_001164042.1	114
	R: 5′ GAAGCTGAGCAAACTCCTGAT 3′	NM_001164041.1; NM_008541.3
Tgfbr1	F: 5′ AAAACAGGGGCAGTTACTACAAC 3′	NM_009370.2	188
	R: 5 ′ TGGCAGATATAGACCATCAGCA 3′
Tgfbr2	F: 5′ AACATGGAAGAGTGCAACGAT 3′	NM_029575.3; NM_009371.3	90
	R: 5′ CGTCACTTGGATAATGACCAACA 3′
Bmpr1a	F: 5′ GGCCATTGCTTTGCCATTATAG 3′	NM_009758.4	109
	R: 5 ′ CTTTCGGTGAATCCTTGCATTG 3′
Bmpr1b	F: 5′ CCCTCGGCCCAAGATCCTA 3′	NM_007560.3	128
	R: 5′ CAACAGGCATTCCAGAGTCATC 3′
18s	F: 5′ CGGCTACCACATCCAAGGAA 3′	NR_003278.1	187
	R: 5′ GCTGGAATTACCGCGGCT 3′

Sftp, surfactant protein; Aqp5, aquaporin 5; Tgf, transforming growth factor; BMP, bone morphogenetic protein; Tgfbr, TGF-β receptor; Bmpr, BMP receptor; F, forward primer; R, reverse primer.